Volume 8 Supplement 1

23rd World Congress of the World Society of Cardio-Thoracic Surgeons: Abstracts

Open Access

Volume-activated chloride channel exists in CD133+ lung cancer stem cells

Journal of Cardiothoracic Surgery20138(Suppl 1):P135

DOI: 10.1186/1749-8090-8-S1-P135

Published: 11 September 2013

Background

The study is to research if volume-activated chloride channel (VACC) existed in tumor stem cells.

Methods

CD133+ cells were purified by magnetic cell separation (MACS) system from non-small cell lung cancer (NSCLC) cell line A549. The purity was detected by flow cytometry. VACC was recorded with whole-cell patch clamp.

Results

The purity of CD133+ cells isolated from A549 by MACS was 92.14%. And VACC was recorded in the 22/40(55%) CD133+ cells of A549 by whole-cell patch clamp.

Conclusion

VACC exist in CD133+ lung cancer stem cells.

Authors’ Affiliations

(1)
Department of Thoracic Surgery, Renmin Hospital, Wuhan University
(2)
Department of Pharmacology, Tongji Hospital, Huazhong University of Science and Technology

Copyright

© Mao and Xu; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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