Fig. 2

SERPINB12 promoted proliferation and tumor growth in NSCLC. (A) Relative protein expression of SERPINB12 in normal epithelial lung cells (16-HBE) and NSCLC cell lines, including H157, H1703, H358, A549, and H1650. (B and C) The knockdown efficacy of SERPINB12 in H358 and A549 cells was verified by qPCR and western blotting. (D-E) CCK-8 assay conducted using SERPINB12-knockdown A549 and H358 cells. (F-G) Colony formation assay conducted using SERPINB12-knockdown A549 and H358 cells. (H) In vivo study to evaluate tumor growth by injecting different cells whose SERPINB12 had been inhibited or not (n = 4 for each group, one-way ANOVA, ** p < 0.01). (I) The tumor weight in the sh-NC and sh-1 groups. (J) IHC staining to evaluate the expression of Ki-67 in the sh-Ctrl and sh-1 groups (scale bar: 200 μm). * p < 0.05, ** p < 0.01, *** p < 0.001