Fig. 3

Knockdown of SERPINB12 impaired the invasion and metastasis of NSCLC cells by inhibiting EMT. (A and B) Wound healing assays conducted using SERPINB12-knockdown and control A549 and H358 cells (scale bar: 50 μm). The percentage of wound closure was calculated at 0 and 24 h. The mean ± SD from three independent experiments are shown. (C and D) Transwell assays were performed with SERPINB12-knockdown and control A549 and H358 cells to examine migration and invasion ability (scale bar: 50 μm). The numbers of cells migrating through the inserts were calculated, and the mean ± SD from three independent experiments are shown. (E) GSEA based on the expression profiles of samples with high and low SERPINB12 expression using the TCGA dataset. (F and G) Relative mRNA and protein expression levels of epithelial markers (BMP2 and CDH1) and mesenchymal markers (CDH2, SLUG, SNAIL, VIM) in A549 cells with or without SERPINB12 knockdown. (H and I) Relative mRNA and protein expression levels of epithelial markers (BMP2 and CDH1) and mesenchymal markers (CDH2, SLUG, SNAIL, VIM) in H358 cells with or without SERPINB12 knockdown. * p < 0.05, ** p < 0.01, *** p < 0.001