Fig. 4

SERPINB12 activated Wnt/β-catenin signaling by facilitating the nuclear translocation of β-catenin. (A) The correlation between SERPINB12 and genes involved in the Wnt signaling pathway. (B) GSEA based on the expression profiles of samples with high and low SERPINB12 expression using the TCGA dataset. (C) The luciferase reporter assay of TOP/FOR was conducted in the sh-NC, sh-1, and sh-2 groups. (D) Nuclear and cytoplasmic expression of β-catenin in the sh-NC, sh-1, and sh-2 groups. (E) The translocation of β-catenin exhibited by immunofluorescence in the sh-NC, sh-1, and sh-2 groups of A549 cells (scale bar: 50 μm). (F) The translocation of β-catenin exhibited by immunofluorescence in the sh-NC, sh-1, and sh-2 groups of H358 cells (scale bar: 50 μm). (G) IHC staining of β-catenin in mouse subcutaneous neoplasia tissue formed by H358 cells treated with sh-NC or sh-1. Scale bar: 200 μm. * p < 0.05, ** p < 0.01, *** p < 0.001