Fig. 5

miR-34b-5p directly targeted the 3’UTR of SRSF1. (A) The putative binding sequence between miR-34b-5p and 3’UTR of SRSF1. (B) Dual-luciferase assay was used in 293 T cells to confirm the binding relationship between miR-34b-5p and SRSF1. (C-D) The level of SRSF1 in NSCLC tissues was detected by qRT-PCR(C) and western blot (D). (E) Correlation between miR-34b-5p and SRSF1 level in 40 NSCLC tissues using Spearman analysis. (F-G) The SRSF1 RNA expression in 16HBE, PC9, HCC827, PC9-GR and HCC827-GR cells was detected by qRT-PCR (F) and western blot (G). *P < 0.05, **P < 0.01, ***P < 0.001