Fig. 6

miR-34b-5p overexpression on gefitinib-resistant NSCLC cell colony formation, apoptosis and gefitinib sensitivity was alleviated by SRSF1. PC9-GR and HCC827-GR cells were transfected with mimic NC, miR-34b-5p mimic, miR-34b-5p mimic + pcDNA or miR-34b-5p mimic + pcDNA-SRSF1. (A-B) SRSF1 mRNA level and protein level in different treated groups were measured by qRT-PCR (A) and western blot (B). (C-E), cell viability, colony formation ability and apoptosis were measured in different treated groups by CCK-8 assay (C), colony formation assay (D) and flow cytometry (E). *P < 0.05, **P < 0.01, ***P < 0.001