Fig. 3

Inhibition of the NLRP3/Caspase-1 pyroptosis pathway reduced TGFβ1-induced CFs transdifferentiation. (A) Western blot to measure protein expression levels; (B) ELISA to determine the expression levels of inflammatory factors; (C) CCK-8 assay to assess cell proliferation; (D) Cell scratch test to evaluate cell migratory rate; (E) Immunofluorescence labelling of α-SMA protein. The experiment was repeated three times. The data were expressed as mean ± standard deviation, with one-way ANOVA used for data comparisons among multiple groups, and Tukey’s test used for post hoc testing. * P < 0.05