Fig. 1
METTL14 downregulation inhibits H/R-induced ferroptosis. HL-1 cells were transfected with si-METTL14, with si-NC as negative control, followed by H/R induction. A: Cell viability was evaluated by the CCK-8 assay; B-D: Fe2+ content (B), ROS levels (C), and GSH levels (D) were determined by assay kits; E: Protein levels of ACSL4, GPX4, and METTL14 were determined by Western blot assay; F: METTL14 mRNA levels were determined by RT-qPCR. Each experiment was repeated three times independently. Data were shown as mean ± standard deviation. Data in panel F (left) were analyzed by the t test, data in panels A-D and F (right) were analyzed by one-way ANOVA, data in panel E were analyzed by two-way ANOVA, followed by Tukey’s multiple comparison test. * P < 0.05, ** P < 0.01