Fig. 4

miR-146a-5p targets and inhibits APPL1 transcription. A: The downstream target genes of miR-146a-5p were predicted on the databases and intersections were identified; B: The binding site of miR-146a-5p and APPL1; C: The binding of miR-146a-5p to APPL1 was analyzed by the dual-luciferase assay; D: mRNA levels of APPL1 in cells were determined by RT-qPCR. Each experiment was repeated three times independently. Data were shown as mean ± standard deviation. Data in panel D were analyzed by one-way ANOVA and data in panel C were analyzed by two-way ANOVA, followed by Tukey’s multiple comparison test. * P < 0.05, ** P < 0.01