Table 1 Primers Design, Sequences and Amplification programs for Polymerase Chain Reaction
From: Possible roles of Epstein-Barr virus in Castleman disease
Target | Sequence (5' to 3') | 1 | 2 | 3 | 4 | 5 | 6 | Â |
|---|---|---|---|---|---|---|---|---|
β-globin |  |  |  |  |  |  |  |  |
   PC03 | ACACAACTGTGTTCACTAGC | 94°C | 94°C | 55°C | 72°C | 72°C | 4°C |  |
   PC04 | CAACTTCATCCACGTTCACC | 5 min | 30 sec | 40 sec | 1 min | 8 min |  | 2~4 35 cycle |
EBV | Â | Â | Â | Â | Â | Â | Â | Â |
   EBNA-2(F) | AGGCTGCCCACCCTGAGGAT | 94°C | 94°C | 56°C | 72°C | 72°C | 4°C |  |
   EBNA-2(R) | GCCACCTGGCAGCCCTAAAG | 5 min | 1 min | 1 min | 1 min | 10 min |  | 2~4 35 cycle |
   EBER(F) | GTGGTCCGCATGTTTTGATC | 94°C | 94°C | 58°C | 72°C | 72°C | 4°C |  |
   EBER(R) | GCAACGGCTGTCCTGTTTGA | 5 min | 30 sec | 1 min | 2 min | 10 min |  | 2~4 35 cycle |