Figure 5

Representative grayscaled fluorescent images of cardiomyocytes treated with cardioplegia and reperfusion (control group, first column) versus the same treatment plus the addition of carvedilol (second column). After DAPI counterstaining the greater nuclei of cardiomyocytes allow their distinction from fibroblasts with smaller nuclei. In PARP-1 cleavage positive, apoptotic cardiomyocytes nuclei feature an intensive granular fluorescence intensity (arrows). The exemplary images represent one experiment with the according cardioplegia and reperfusion time set noted on the left side. During the cryosection procedure artifacts presenting as nuclei conglomerates could not be avoided; these were excluded from analyses.