Antiapoptotic effect of cardioplegia versus ischemia. The x-axis represents time in min and the y-axis the number of PARP-1 cleavage positive, apoptotic cardiomyocytes. There is a time-dependent significant (p < 0.05) increase of apoptotic cardiomyocytes after reperfusion. Further there is a significant (p < 0.05) reduction of apoptosis in cardiomyocytes treated with cardioplegia versus ischemia. One column represents data of n = 5 experiments, noted as mean ± SEM. Stars mark the significances between the compared columns. The mean total cardiomyocyte number in 3 analyzed central areas on the cryosections was 300 ± 25 (mean ± SEM).