Table 1 Primer pairs that have been used in PCR
From: Bacterial and fungal microflora in surgically removed lung cancer samples
Organism | Species | Forward Primer (5'-3') | Reverse Primer (5'-3') | PCR Product (bp) |
|---|---|---|---|---|
Treponema | pallidum | AATGCGGTGGCGTAGCGATAC | TTTTGCGGTTTGCTCCACTTC | 275 |
| Â | denticola | AGGGATATGGCAGCGTAGCAATA | CGTCCTCCCTTACGGGTTAGACT | 453 |
| Â | vincentii | GCGGTATGTAAGCCTGGTGTGAA | TTTGCTTTGGCACTGAAGCTCTT | 277 |
Neisseria | meningitidis | AAGTCGGACGGCAGCACAGA | TCAGCCGCTGATATTAGCAACAG | 421 |
Legionella | pneumophila | AAGATTAGCCTGCGTCCGATTAG | AACCCTCCTCCCCACTGAAAGT | 232 |
Borrelia | burgdorferi | CATGCAAGTCAAACGGGATGTA | GACCTTCTTCATTCACGCAGTG | 361 |
| Â | americana | Â | Â | Â |
| Â | valaisiana | Â | Â | Â |
| Â | garinii | Â | Â | Â |
| Â | recurrentis | Â | Â | Â |
| Â | hispanica | Â | Â | Â |
| Â | duttonii | Â | Â | Â |
| Â | lusitaniae | Â | Â | Â |
| Â | spielmanii | Â | Â | Â |
Listeria | grayi | TCTTGACATCCTTTGACCACTCTG | TGCACCGGCAGTCACTTTAGAG | 157 |
| Â | innocua | Â | Â | Â |
| Â | monocytogenes | Â | Â | Â |
| Â | welshimeri | Â | Â | Â |
Helicobacter | pylori | GATTGGCTCCACTTCGCAGTA | GGCGACCTGCTGGAACATT | 560 |
| Â | pullorum | Â | Â | Â |
| Â | equorum | Â | Â | Â |
| Â | canadensis | Â | Â | Â |
Staphylococcus | aureus | AGGCGACTTTCTGGTCTGTAACTG | CCGAAGGGGAAGGCTCTATCT | 307 |
Haemophilus | parasuis | CCTTGGGAAAATACTGACGCTCAT | TCCCGAAGGCACACTCTCAT | 297 |
Chlamydia | muridarum | TGTTTAGTGGCGGAAGGGTTAG | CCGTCCATTGCGAAAGATTC | 304 |
| Â | trachomatis | Â | Â | Â |
Bacillus | pumilus | TGCAAGTCGAGCGGACAGA | TCCCAGTCTTACAGGCAGGTTAC | 91 |
| Â | aerophilus | Â | Â | Â |
| Â | licheniformis | Â | Â | Â |
| Â | amyloliquefaciens | Â | Â | Â |
| Â | subtilis | Â | Â | Â |
Bacillus II | anthracis | CGGCTTCGGCTGTCACTTATG | TCAGCACTAAAGGGCGGAAAC | 655 |
| Â | cereus | Â | Â | Â |
| Â | thuringiensis | Â | Â | Â |
Mycoplasma | pneumoniae | GAGGCGAACGGGTGAGTAACA | CGCGACTGCTGGCACATAGT | 441 |
| Â | pirum | Â | Â | Â |
| Â | gallicepticum | Â | Â | Â |
| Â | genitalium | Â | Â | Â |
| Â | amphoriforme | Â | Â | Â |
Leptospira | borgpetersenii | GGATAGCCCCGAGAGGTCATA | CCATCATCACATCGCTGCTTAT | 299 |
Leptospira | meyeri | CGAATGTGACGGTTCCTGGTAG | TTCGCCCATTGAGCAAGATT | 210 |
| Â | biflexa | Â | Â | Â |
Staphylococcus | epidermidis | GTGAAAGACGGTTTTGCTGTCAC | CGGATAACGCTTGCCACCTAC | 359 |
Streptococcus | mitis | GGAGCTTGCTCTTCTGGATGAG | GAGCCGTTACCCCACCAACT | 197 |
Leptospira | interrogans | CAGCCTGCACTTGAAACTATGTG | ATAGTCCCCAGGCGGTCTACT | 266 |
Brachyspira | hyodysenteriae | TGCCGTAGAGTGGGGGATAA | CCGCAGGCTCATCGTAAAG | 109 |
| Â | aalborgi | Â | Â | Â |
| Â | intemedia | Â | Â | Â |
| Â | alvinipulli | Â | Â | Â |
| Â | innocens | Â | Â | Â |
| Â | suanatina | Â | Â | Â |
Haemophilus | influenzae | CTTGCTTTCTTGCTGACGAGTG | TCTCAGTCCCGCACTTTCATC | 129 |
Candida I | albicans | CCAGCCGAGCCTTTCCTTCT | TACCCCCGACCGTCCCTATT | 187 |
| Â | parapsilosis | Â | Â | Â |
| Â | dubliensis | Â | Â | Â |
Candida | tropicalis | CGGTCGGGGGTATCAGTATTC | ATACTCGCTGGCTCCGTCAGT | 622 |