Figure 1

Prolonged cold ischemia leads to earlier lesion formation. Aortic allografts were exposed to 20 min cold ischemia (left panel) or 60 min cold ischemia (right panel) and harvested between 3 wk and 6 wk. Representative sections from elastin stained aortic allografts harvested at (a) and (b) 3 wk post-transplant; (c) and (d) 4 wk post-transplant; (e) and (f) 6 wk post-transplant; (g) lesion size as mean ± SEM. n = 4 animals per time point. Orange arrows point to internal elastic lamina.