Figure 2
From: Early innate immune events induced by prolonged cold ischemia exacerbate allograft vasculopathy
Medial smooth muscle cell recovery is hindered in grafts exposed to prolonged cold ischemia. Aortic allografts were exposed to 20 min cold ischemia (white bars) vs. 60 min cold ischemia (black bars) and harvested between 1 d-6 wk. A control group of native, non-transplanted aortic sections were removed and immediately processed for histology (stippled bar). Three randomly chosen sections were selected from each graft for digital image analysis to enumerate medial SMC numbers. Data shown here represents mean ± SEM. n = 4 animals per time point.