Figure 4From: Early innate immune events induced by prolonged cold ischemia exacerbate allograft vasculopathy Grafts exposed to prolonged cold ischemia have a higher percentage of proliferating medial SMC. Aortic grafts exposed to 20 or 60 min of cold ischemia were harvested at 2 wk post transplant and stained using Ki-67 to assess proliferation. Ki-67 positive SMC were enumerated on three representative sections per graft. The percentage of proliferating SMC was calculated using the number of SMC enumerated on H&E sections. Data shown here represents mean ± SEM. n = 4 animals per time point.Back to article page