Figure 1

Microperfusion chamber. The perfusate enters the chamber, constructed from plexiglas (2), through the pipe (1) and fills the rectangular shaped chamber (3). Once laminar flow is constituted the cardiac tissue is physically fixed before the nylon net (not featured), which spans in a 135° angle. The fluid exits on the opposite side (4). Between the bottom and the upper part of the chamber a rubber layer was placed for sealing and fastened with 4 screws.