Evidence of generation of regulatory cells in CBA allograft recipients exposed to opera music. A,B,C, Cardiac allograft survival after adoptive transfer of whole splenocytes (A), CD4+ cells (B), or CD4+CD25+ cells (C). MST, median survival time. #P < 0.01, ##P < 0.005 and ###P < 0.001 for difference between two groups. D, Forkhead box P3 (Foxp3)+ cells with use of double immunostaining. In Figure 2D, the left-hand panels show samples obtained from mice exposed to opera music (magnification × 40 and × 100) and the right-hand panels show samples from untreated mice (magnification × 40 and × 100). E, CD4, CD25, and Foxp3 expression in splenocytes as determined by flow cytometry 1, 2, and 4 weeks after transplantation. *P > 0.05, ##P > 0.005 and ###P < 0.001 for difference between two groups. NS, not significant.