Table 1 PCR primer of 14 ECM-related gene
Primer name | Sequence (5’ → 3’) | Length of product |
|---|---|---|
COL4A4/F | ACCCTGCCAGTCACTTTGGTC | 103bp |
COL4A4/R | ATACCAGGCAAGCCCTGCTC | |
COL11A1/F | AGCTGCAGGCCAAGATGGA | 116bp |
COL11A1/R | CAATCAGGCCAATTAAACCAGGA | |
FN-1/F | GAGCTGCACCTGTCTTGGGAAC | 133bp |
FN-1/R | GGAGCAAATGGCACCGAGATA | |
TNC/F | ACTCGCTACAAGCTGAAGGTGGA | 122bp |
TNC/R | GCACAGTTGGTGATGGATGAA | |
THBS/F | TGCTCCAATGCCACAGTTCC | 132bp |
THBS/R | CTGCTGAATTCCATTGCCACA | |
FBLN/F | CAACCTGCAGCAGCAGACGTGCTA | 125bp |
FBLN/R | AGCCAGGGTTCTCAGCAGGA | |
MMP3/F | GGGTGAGGACACCAGCATGA | 178bp |
MMP3/R | CAGAGTGTCGGAGTCCAGCTTC | |
MMP9/F | ACGCACGACGTCTTCCAGTA | 94bp |
MMP9/R | CCACCTGGTTCAACTCACTCC | |
TIMP-3/F | TGGCCAAGCTGGAGGTCAAC | 75bp |
TIMP-3/R | CCCGTGTACATCTTGCCATCATAG | |
WNT5A/F | TTCGCCCAGGTTGTAATTGAAG | 168bp |
WNT5A/R | CTGCATGTGGTCCTGATACAAGTG | |
SGCD/F | CTTGGCCATGACCATCTGGA | 132bp |
SGCD/R | GGCAGACTTGAAGTACAGGGCATTA | |
COL14A1/F | AAGCCCAGAGTCAAAGTTGTGGA | 123bp |
COL14A1/R | CCATGAACCATCGACCAGGA | |
ELN/F | AGCAAGACCTGGCTTCGGATT | 122bp |
ELN/R | CCAACGTTGATGAGGTCGTGA | |
PLAT/F | TCGAGACTCAAAGCCCTGGTG | 119bp |
PLAT/R | AGGCTGACCCATTCCCAAAGTAG | |
β-actin/F | CAACTGGGACGACATGGAGAAA | 178bp |
β-actin/R | GATAGCAACGTACATGGCTGGG |  |