Standard curves of miR-133a, miR-208a, and miR-133b for TaqMan qRT-PCR assays. Standard curves were generated for each miRNAs assay using a dilution series of known input amounts of synthetic miRNAs oligonucleotide corresponding to the target of the assay (Table 1). Data points not on the trend line are those for which the measured Ct was interpreted to be below the linear range of the assay, and were not used for derivation of the trend line. A, Standard curve of miR-133a; B, Standard curve of miR-208a. C, Standard curve of miR-133b.