Figure 7
Effect of SEA0400 in cardioplegia on troponin release and mitochondrial morphology in isolated hearts. A) Troponin release was measured by ELISA assay from coronary sinus effluent during reperfusion. B) Relative number of intact, swollen, and disrupted mitochondria was quantified from electron micrographs of isolates from LV myocardium after cardioplegic arrest and reperfusion. Mitochondria isolated from hearts not subjected to ischemic cardioplegic arrest (Normal) were analyzed for comparison. Bars represent mean ± SEM, * = p = 0.0026 for cardioplegia without SEA0400 vs. cardioplegia with SEA0400, Ф = p < 0.001 for Normal vs. cardioplegia without SEA0400, ** = p < 0.05 for disrupted mitochondria (cardioplegia without vs. with SEA0400), *** = p < 0.01 for intact mitochondria (cardioplegia without vs. with SEA0400), n = 6 (Normal & cardioplegia without SEA0400), and 7 (cardioplegia with SEA0400).