Experimental design. A: Experiment 1; An airtight circuit is formed from a pressure gauge, a plastic syringe, and a pressure-hardened plastic tank covered by rabbit skin with 5 holes. The holes are covered with TachoComb sheets (arrow). B: Experiment 2; A silk suture loop was placed on the pericardium and fixed with a TachoComb sheet plus a PGA sheet and fibrin glue (arrows). After stabilization, tension was gradually applied to the loop with a digital gauge.