Evidence of generation of regulatory cells in CBA recipients of cardiac allografts given olfactory exposure to TJ-23. Allograft survival times after adoptive transfer of whole splenocytes (A), CD4+ splenocytes (B), or CD4+CD25+ splenocytes (C). MST, median survival time; *P < 0.01 for difference between two groups. (D) CD4, CD25, and Foxp3 expression in splenocytes, as determined by flow cytometry. Data are mean ± SD values for the percentage of CD4+CD25+Foxp3+ in CD4+ cells in samples from five mice in each group. *P < 0.01 for difference between 2 groups. (E) Foxp3+ cells detected by double immunostaining in cardiac allografts obtained 30 days after transplantation from mice given olfactory exposure to TJ-23 and untreated mice (magnification × 40).