Figure 2From: Fox smell abrogates the effect of herbal odor to prolong mouse cardiac allograft survival Evidence of generation of regulatory cells in CBA recipients of cardiac allografts given olfactory exposure to TJ-23. Allograft survival times after adoptive transfer of whole splenocytes (A), CD4+ splenocytes (B), or CD4+CD25+ splenocytes (C). MST, median survival time; *P < 0.01 for difference between two groups. (D) CD4, CD25, and Foxp3 expression in splenocytes, as determined by flow cytometry. Data are mean ± SD values for the percentage of CD4+CD25+Foxp3+ in CD4+ cells in samples from five mice in each group. *P < 0.01 for difference between 2 groups. (E) Foxp3+ cells detected by double immunostaining in cardiac allografts obtained 30 days after transplantation from mice given olfactory exposure to TJ-23 and untreated mice (magnification × 40).Back to article page