Fig. 1

Alpha-gal ELISA assay- Relative concentrations of α-gal epitopes were calculated based on fresh porcine cusp defined as 100%. Decellularized cusps had 2.8 ± 2.0% relative α-gal epitope while human aortic cusps had 2.0 ± 0.4% as compared to fresh porcine aortic valve cusps. The relative concentration of α-gal epitope in the decellularized cusps was not statistically significantly different (p = 0.4) from the human aortic cusp