Fig. 2

Anti-pig antibody staining- Detection of anti-pig antibodies in sheep serum. a. Sheep serum taken preoperatively showed no evidence of anti-pig antibodies and as such there is no green or red fluorescence. The blue is auto-fluorescence of decellularized porcine valve with the addition of DAPI. b. Sheep serum taken at 1 week postoperatively shows an abundance of anti-pig IgM antibodies (green) and evidence of anti-pig IgG antibodies (red). The autoflorescence of decellularized porcine valve with the addition of DAPI (blue) is also seen. c-d. Sheep serum taken at 1 month and 2 months postoperatively shows an abundance of anti-pig IgM (green) and IgG (red) antibodies. e-h. Sheep serum at each time point was incubated with decellularized ovine valves. There is no evidence on any IgM (green) or IgG (red) antibodies. The addition of DAPI appears as autoflourescene of decellularized ovine valves. This was a negative control to show that antibody binding was specific to porcine tissue