Fig. 4

Optimization of the talc-induced pericardial adhesion method. Talc was injected at a low dose (500 μL of 2.5 mg/g) or high dose (500 μL of 5.0 mg/g) into the murine pericardial cavity. After 1, 2, and 4 weeks, the adhesive grade was calculated by scoring the strength and volume of adhesion tissues. a Representative heart photos from each scored mouse. The broken line indicates the heart surface area. b Relationship of adhesion score and treatment term (by week) following low-dose (left panel, 2.5 mg/g) and high-dose (right panel, 5.0 mg/g) injection of talc (n = 4). Categories A and B represent the volume and strength of adhesion tissues, respectively