Fig. 4

Gli signaling promotes cell migration and invasion in NSCLC cell lines. a Wound healing assays of A549 lung cancer cells, treated with DMSO (control), N-Shh (0.5 mg/mL), GANT61(15umol/L), or GANT61(15umol/L) with N-Shh (0.5 mg/mL) stimulation. Representative images captured by a light microscope are shown (100x). b Quantification of wound healing assays, expressed as percentage of wound in comparison to 0 h (p-values of < 0.01 or < 0.001 were indicated as ** or ***, respectively). c Transwell invasion assay of A549 lung cancer cells, treated with DMSO (control), N-Shh (0.5 mg/mL), GANT61(15umol/L), or GANT61(15umol/L) with N-Shh (0.5 mg/mL) stimulation. Representative images captured by a light microscope are shown (100x), average of 5 picture fields at 100x total magnification. d Quantification of transwell invasion assays, expressed as percentage of wound in comparison to 0 h (p-values of < 0.01 or < 0.001 were indicated as ** or ***, respectively)