Fig. 4
From: Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis
EIF4E is the downstream target of miR-499a-5p. a Through the RNA22, Starbase and TargetScan, the target genes of miR-499a-5p were screened by Venny map; b the binding sites of miR-499a-5p and EIF4E were predicted by the Starbase; c dual-luciferase assay verified that miR-499a-5p combined with EIF4E; d the expression of EIF4E mRNA in sepsis patients and healthy volunteers, survival group and non-survival group was detected by RT-qPCR (N = 60); e the correlation between miR-499a-5p and EIF4E was analyzed; f the expression of EIF4E mRNA in AC16 cells was detected by RT-qPCR; The data of c and f were depicted as mean ± SD. Two-way ANOVA was used in figure c and one-way ANOVA was used in figure f, followed by Tukey's post hoc test, and independent t test was used in figure d. *P < 0.05