Fig. 5
From: Predictive value and regulatory mechanism of serum miR-499a-5p on myocardial dysfunction in sepsis
Overexpression of EIF4E reverses the role of miR-499-5p in SIMD. AC16 cells treated with LPS and transfected with miR-499a-5p-mimic were transfected with oe-NC or oe-EIF4E. a The expression of eIF4E mRNA was detected by RT-qPCR; b CCK-8 was used to detect cell viability; c the expressions of CK-MB, BNP, SOD and COX IV were detected by ELISA. Three independent repeated experiments were carried out, and the data were depicted as mean ± SD. T test was used for data analysis in panel a, two-way ANOVA was used for panel b, and one-way ANOVA was used for panel c, followed by Tukey’s post hoc test. *P < 0.05