Fig. 3

CircMMP11 overexpression decreased miR-143 expression via methylation. To study the crosstalk between circMMP11 and miR-143, Calu-3 and H2170 cells were transfected with either circMMP11 expression vector or miR-143 mimic. Overexpression levels of circMMP11 and miR-143 were monitored every 24 h until 144 h (A). The effect of circMMP11 expression vector transfection on miR-143 expression (B) and the effect of miR-143 mimic transfection on circMMP11 expression (C) were analyzed by RT-qPCRs. The effect of circMMP11 on miR-143 gene methylation was analyzed by MSP (D). U, unmethylaion; M, methylation. *p < 0.05