Fig. 6

Nesfatin-1 alleviated oxidative stress in LPS-stimulated RAW 264.7 cells. Cells were treated with LPS (1 μg/mL) in the presence and absence of Nesfatin-1 (50, 100 nM). (A) ROS level was detected using an intracellular fluorophore (DCFH-DA) assay; (B) Activity of SOD was detected using SOD activity assay kit (***P < 0.0001 vs. vehicle group; #, ##, P < 0.01, 0.001 vs. LPS treatment group)