Fig. 5
From: A modified primary culture method of rat pulmonary vein smooth muscle cells
Identification of PVSMCs by morphology and immunofluorescence
a, b, c: A large amount of α-SMA was observed under a fluorescence microscope. The purity of PVSMCs was determined to be high based on the association between α-SMA (stained red) and the cell nucleus (stained blue). d, e, f: The immunofluorescence results showed that α-SMA was negative in the control PVSMCs