A rare case of Candida parapsilosis endocarditis in a young healthy woman – case report

Abstract Disseminated fungal infections are still rare conditions, mostly caused by Candida spp. during immunosuppression. Infection of immunocompetent individuals is uncommon. Endocarditis is a rare manifestation during candidaemia, mostly in patients with prosthetic valves. Affection of previously unaltered valves is uncommon. Case presentation We presented a case of a young, previously healthy female patient with endocarditis, caused by Candida parapsilosis. The initial symptom, fever, was present four months before hospital admittance. She was febrile without other symptoms and during observation in a local hospital. After her condition deteriorated, she was transferred to the Institute for infectious and tropical diseases, Belgrade. Clinical findings on admission include petechial skin rash and moderate hepatosplenomegaly. Newly developed systolic murmur was noted, and Candida parapsilosis was isolated in multiple blood cultures. Echocardiography revealed 15 × 14 mm vegetations on the right aortic vellum. She was treated with antifungal drugs (fluconasole, liposomal amphotericin B), and the affected valve was successfully replaced. The same strain of Candida parapsilosis was isolated from the intraoperative material of the valve. There were no markers of immunosuppression or other conditions which could affect the immune system. Conclusion After a prolonged period of treatment she was successfully cured, and she received a long-term intermittent suppressive fluconasole therapy for the time being.


Background
Although disseminated fungal infections are more common today than before, they still remain rare conditions, mostly caused by Candida spp. Candida parapsilosis and Candida tropicalis are the most common causes in Europe [1,2]. Risk factors include immunosuppression (HIV, neutropenia, transplants, solid tumors), but infection of immunocompetent individuals is uncommon [1,3]. Candidaemia has a significant mortality rate, up to 44% [1,2].
Endocarditis is present in 5-25% of patients with candidaemia, mostly in patients with prosthetic valves [4,5]. Affection of previously unaltered heart valves is uncommon. During the past 20 years, there have been only three cases reported in Sweden [4]. Other authors also refer to Candida endocarditis as an extremely rare occurrence in patients with normal native cardiac valves [6,7].

Case presentation
We will present a case report of a previously healthy twenty-three year old Caucasian female patient, from a higher social-culture level, living in Belgrade suburbs. She had a negative history data to preexisting diseases, drug use or any underlining conditions. First symptom-fever, presented four months before hospital admittance, was treated with third generation cephalosporin (ceftriaxone 2,0 gr daily i.v.) and resolved within 5 days. During the next month she was febrile without other symptoms. During that time the patient was observed in a local hospital, until her condition deteriorated -with anemia, leucopenia and petechial skin rash, and she was transferred to the Institute for Infectious and Tropical Diseases, Belgrade, Clinical Centre of Serbia. Clinical findings on admission included discreet petechial skin rash, moderate hepatosplenomegaly, whereas other systems were unaffected, including normal heart sounds. Laboratory tests showed moderate elevation of erythrocyte sedimentation rate, intermediary anemia and leucopenia, with elevated C-reactive protein. (Table 1) Candida parapsilosissensitive to all systemic antifungal drugs was isolated in multiple blood cultures. The initial treatment included IV fluconazole 200 mg/12 h. During the third day of therapy, a newly developed systolic murmur was noted, presenting an indication for echocardiography. The patient was afebrile starting from that moment.
Echocardiography revealed 15 × 14 mm vegetations on the right aortic vellum. (Figure 1) As Candida endocarditis usually appears in immunosuppressive patients as well as in patients with prosthetic valves, extensive clinical investigation was performed, to uncover any possible cause of immunosuppression. During a four-week period, all possible infectious agents were tested, including HIV, HBV, HCV, EBV, Leischmania and tuberculosis. Normal absolute count and ratio of CD4, CD2, CD3 and CD8 lymphocytes was noted, as well as normal concentration of immunoglobulines and sufficient immunological functions. There were no markers of autoimmune diseases which could affect the immune system. Hematological investigation was performed, as well as abdominal and chest CT, so the possible presence of solid tumors was excluded. A "10-panel urine screen" drug test was negative. During investigation, the patient was treated with fluconazole (200 mg/12 h, i.v.). Control echocardiography showed progressive enlargement of vegetations, spreading to the other vellum, so the treatment was continued with liposomal amphotericin B intravenously, 50 mg daily. Since the transoesophageal echocardiography showed that after two weeks of new treatment there were two additional vegetations affecting vellums (17 × 6 mm and 12 × 3 mm), it was necessary to replace the affected heart valve. Control blood cultures, repeated daily from the fifth day of hospitalization (patient was afebrile), were sterile. Preoperatively, patient was treated with fluconazole for 38 days, and liposomal amphotericin B for 15 days. The affected valve (Figure 2) was successfully replaced, and the same strain of Candida parapsilosis was isolated from intraoperative material of the valve.
Further conservative antifungal therapy was administered after operation, until laboratory findings were normalized, five months later. During this period the patient was treated with liposomal amphotericin B for 36 days, IV in the daily dose of 50 mg. For the rest of the period she was treated with fluconazole IV 200 mg in the dose intervals of 12 hours (approximately four months). During the whole period, no side-effects were observed.
During the 3 year follow-up, there were no other pathological developments, and the patient has been physically active and working.

Discussion
This report presents a rare disease progress in a young and previously healthy person, without any predisposing conditions -no immunodeficiency or previous valve defect [4][5][6]. In spite of intensive systemic antifungal therapy, which is recommended [2,4,5] there was no improvement, so the operative valve replacement was necessary, which has also been reported in other cases [4][5][6][7].
After the patient was discharged from the hospital, she received a long-term intermittent suppressive fluconasole therapy. There is some difference in opinion concerning the time length of this therapy, including possibility of life-long treatment [5,8,9]. During the three year follow-up, our patient has been taking 200 mg of fluconasole twice a week with complete recovery and no other physical complaints. Legend: *SE-erythrocytes sedimentation rate during first hour, HgBhemoglobin, Erery throcytes, Leleucocytes, Trthrombocytes, CRP -C-reactive protein, PTprothrombine time.