Fluorescence in situ hybridization as adjunct to cytology improves the diagnosis and directs estimation of prognosis of malignant pleural effusions
- Jingquan Han†1,
- Shouqiang Cao†1,
- Kai Zhang1,
- Guibin Zhao1,
- Yanzhong Xin1,
- Qing Dong1,
- Yubo Yan1 and
- Jian Cui1Email author
© Han et al.; licensee BioMed Central Ltd. 2012
Received: 26 March 2012
Accepted: 3 November 2012
Published: 13 November 2012
The identification of malignant cells in effusions by conventional cytology is hampered by its limited sensitivity and specificity. The aim of this study was to investigate the value of fluorescence in situ hybridization (FISH) as adjuncts to conventional cytologic examination in patients with malignant pleural effusions.
We conducted a retrospective cohort study of 93 inpatients with pleural effusions (72 malignant pleural effusions metastatic from 11 different organs and 21 benign) over 23 months. All the patients came from Chinese northeast areas. Aspirated pleural fluid underwent cytologic examination and fluorescence in situ hybridization (FISH) for aneuploidy. We used FISH in single-colour or if appropriate in dual-colour evaluation to detect chromosomal aberrations (chromosomes 7, 11, and 17) in effusion cells as markers of malignancy, to raise the diagnostic yield and identified the efficiency by diagnostic biopsy. Predominant cytogenetic anomalies and patterns of intratumor cytogenetic heterogeneity were brought in relation to overall survival rate.
Cytology alone confirmed malignant pleural effusions in 45 of 72 patients (sensitivity 63%), whereas FISH alone positively identified 48 of 72 patients (sensitivity 67%). Both tests had high specificity in predicting benign effusions. If cytology and FISH were considered together, they exhibited 88% sensitivity and 94.5% specificity in discriminating benign and malignant effusions. Combined, the two assays were more sensitive than either test alone. Although the positive predictive value of each test was 94.5%, the negative predictive value of cytology and FISH combined was 78%, better than 47% and 44% for FISH and cytology alone, respectively. There was a significantly prolonged survival rate for patients with aneuploidy for chromosome 17.
FISH in combination with conventional cytology is a highly sensitive and specific diagnostic tool for detecting malignant cells in pleural effusions . The high sensitivity and specificity may be associated with geographic area and race. Simple numeric FISH anomalies may be prognostic.
KeywordsMalignant pleural effusions FISH Prognosis Sensitivity Specificity
Diagnosis of malignancy in pleural effusions from cancer patients is frequently troublesome for the cytologist because the differentiation of malignant cells from accompanying elements such as atypical reactive mesothelial cells is difficult . On the other hand, in pleural effusions tumour cells may appear quite similar to normal cells, for example, small-cell lung cancer cells and lymphocytes . Due to these difficulties, cytopathologists traditionally adopt a rather cautious approach in the diagnosis of malignancy in effusions. With a negative cytology result in a patient with suspected malignant pleural effusion, standard clinical practice involves a repeat thoracentesis, a pleural biopsy, or thoracoscopy to prove the presence of tumor cells in the pleural fluid. Thoracoscopy is effective in this regard and has a high diagnostic yield for malignant disease involving the pleura . Nevertheless, the risks of additional diagnostic procedures that are painful and invasive, such as thoracoscopy, present a considerable clinical challenge. Then new methods complementing cytology for the diagnostic work-up of pleural effusion need to be evaluated.
Genomic alterations are a hallmark of malignant cells. In the resent years, interphase cytogenetics by fluorescence in situ hybridization (FISH) has been increasingly used in clinical pathology to delineate chromosomal aberrations in neoplasia [4–8]. Because carcinoma cells are regularly characterized by extensive chromosomal aberrations, FISH analysis can be used as a diagnostic tool to detect aneuploid cells characterizing malignancy, provided that cutoffs for background nondisomy are considered. We performed tumour-associated aneuploidy analyses on prospectively collected pleural fluids in order to investigate if there was a complimentarity of cytology and chromosomal aberrations assays in detecting malignant tumor cells in the fluids. Overall survival rate from the first diagnosis of malignancy until death or final follow-up was evaluated. We hypothesized that FISH would be able to detect patients with malignant chromosomal aberrations in their pleural fluid and that, used with cytology, they would enhance diagnosis. Moreover, FISH may be associated with survival rate in patients with malignant pleural effusions.
Clinical and pathologic features of the study population
Effusion sampling and investigation analyses had been approved by the institutional ethics committee. The study population comprised 93 patients with documented pleural effusions who underwent pleural fluid aspiration for diagnostic purposes at The Fourth Affiliated Hospital of Harbin Medical University between October 2008 and September 2011. All the patients were born in Chinese north areas. The study was presented to the Hospital Ethical Board and accepted as this is an observational study based on the best available evidence. The research was conducted conformed to the Helsinki Declaration and to local legislation. Patients gave informed consent to participate in the study. Results of conventional cytology and FISH analyses of the pleural fluids from patients were compared with respect to the definitive diagnosis established by either tissue biopsy, or through clinical follow-up in the case of patients with benign disease. Patients who were free of malignancy had a median follow-up of 6.5 months (range, 1.2 to 32.4). The median age of the study population was 68 years (range, 23 to 85) and there was a predominance of males (63 of 93, or 68%). Pleural invasion was defined as invasion of tumor through either the parietal or visceral pleura.
Pleural fluid was obtained either via a needle during thoracentesis (42%), a chest tube during thoracostomy (13%) or by aspiration through a 1-cm incision at the very beginning of a thorascopic pleurodesis procedure (45%). Aspirated pleural fluid was collected in sterile tubes without anticoagulant and rapidly brought to The Fourth Affiliated Hospital of Harbin Medical University pathology laboratory for conventional cytologic examinations as well as to the research laboratory where they were analyzed by FISH.
About 100 cc of aspirated pleural fluid was spun in a centrifuge at 2,160 rpm for 10 minutes, supernatant removed, and the cell pellet both preserved onto glass slides in ethanol for Papnicolau staining as well as in formalin and ethanol for processing into cellblocks as per standard protocol in the hospital pathology laboratory. The presence or absence of malignant cells in the cytologic material was reported with the agreement of two attending cytopathologistes.
Fluorescence in situ hybridisation analysis
Cells of at least 200 mL of effusion fluid were gained by centrifugation, and, in case of macroscopic blood contamination, subjected to density gradient separation over Ficoll-Hypaque (Sigma, St Louis, MO). Pelleted effusion cells were washed in phosphate-buffered saline, fixed in methanol-acetic acid (3:1, v/v) and stored at −80°C. Directly fluorescence-labeled alpha-satellite DNA probes (SpectrumGreen [excitation peak of 497 nm, emission peak of 524 nm] and SpectrumOrange [559 nm/588 nm]; Vysis Inc, Downers Grove; IL) were applied in dual-color FISH experiments. The probes used in this study were specific for the centromeres of chromosomes 7, 11, and 17. The standard protocol followed was described in a previous report .
Fluorescence microscopy and definition of cutoffs for aneuploidy
Cutoff threshold levels for background aneusomy (percentage) for the three chromosomes examined
Signal number category
Sensitivity, specificity, positive predictive values and negative predictive values of both FISH and conventional cytology as well as of the combination of both techniques were calculated in relation to the definitive diagnosis of the patients in the study. Correlation between variables was estimated using the Fisher’s exact test, or the χ2 test when appropriate. Survival time was analyzed using the Kaplan-Meier method, and differences between two groups(exhibiting or not exhibiting aneuploidy for chromosome 17) were evaluated with the log-rank test. All P values are two-sided, and all significant associations were considered when the P value was 0.05 or less. SSPS16.0 software was used for calculations.
Definitive Diagnosis of Patients in Study
Type of Primary Cancer, n=93
Nonsmall cell lung
Sensitivity, positive predictive value, and negative predictive value of conventional cytology, fluorescence in situ hybridisation, and the tests combined
Correlation of cytology and FISH analyses of pleural fluid
Although both cytology and FISH analysis were comparable in terms of sensitivity, the tests were independent of each other. FISH identified 30 of 45 pleural fluid aspirates (67%) that were positive by cytologic analysis. In addition, Fluorescence in situ hybridisation detected the presence of chromosomal aberrations in the fluid from 18 of 27 patients (67%) who had cytology negative aspirates. In 9 patients (1 patient with acute myelocytic leukemia, 1 with renal cell carcinoma, 2 with ovarian carcinoma, 2 with skin carcinoma, and 3 with NSCLC), cytology and FISH as a combined analysis failed to detect malignant cells or chromosomal aberrations, respectively. FISH had a better sensitivity in predicting the presence of a malignant pleural effusion from a NSCLC primary cancer than cytology (30 of 39, or 77%, versus 24 of 39, or 62%, respectively). Of those patients with breast cancers, cytology and FISH both were able to detect malignant pleural effusions with sensitivities of over 90%.
Transudative versus exudative pleural fluid
Data concerning the character of the pleural effusions (transudative or exudative) was available on 69 patients (74%). A transudate was defined by a protein concentration of <2.5 g dl-1. There were six transudative effusions in the patients with a primary malignancy and both cytology as well as molecular examination did not discern in these specimens any malignant cells or chromosomal aberrations respectively. There were also 51 exudative effusions in all, 9 of which were in patients with no malignancy. The combined tests of FISH and cytology were more accurately able to classify the exudative effusions as being malignant (33 of 42, or 79%) than either cytology (24 of 42, or 57%) or FISH alone (27 of 42, or 64%).
Analysis of prognosis of patients with malignant pleural effusions
Molecular analysis of the pleural fluid of patients using fluorescence in situ hybridisation to discriminate those with benign and malignant pleural effusions has not been well studied. We hypothesized that in patients in whom malignant cells may not be detectable in the pleural fluid by conventional cytology, tumour-associated aneuploidy would be present in sufficient quantities to be detected, and that the sensitivity of this approach would improve the overall diagnostic sensitivity of conventional cytology. Our data show that even for multiple tumor types, and with a limited cohort size, we were able to improve the diagnostic performance of cytologic analysis by measuring tumour-associated aneuploidy in the pleural fluid. We employed a molecular technique and observed that conventional cytology and FISH analysis alone have similar sensitivities (63% and 67%, respectively), but that the sensitivity improves to 88% when the results of the FISH and cytology techniques are combined. It is noteworthy that cytology analysis cost $60 for each case vs $625 of cytology analysis combined with FISH. It has been proposed that the use of tissue microarray for FISH might provide satisfactory results with a markedly cost reduction.
Tumor cell detection in effusions can be significantly improved by FISH and PCR techniques applying appropriate molecular markers. PCR method targeted at tumor-specific genetic abnormalities could detect a small number of cancer cells mixed within a large number of normal cells. This method offered considerable promise for early diagnosis of malignant pleural effusions and detection of tumor progression before clinically evident metastasis. However, the clinical application has been limited so far. The primary limitation had been the lack of suitable target genes common to most tumors. The other limitation of RT-PCR is related to laboratory method per se.
FISH analysis for the diagnosis of malignancy is based on the fact that tumor cells are regularly chromosomally aberrant, mostly harboring complex polysomies for one or multiple chromosomes. The methodical approach used here is based on the previous observation that the identification and quantification of aneuploidy by FISH can be used as a sensitive and highly specific marker of malignancy in metastatic cells [11, 14–17]. We selected chromosomes 7, 11, and 17 for this study, which were found to be prognostic markers in malignant tumour [18–21]. We chose to use a combination of probes to these 3 chromosomes as the basis for our FISH test to detect genetically abnormal pleural effusions. Although we had tumors from 11 different organ sites, we designed a broad panel of genes to detect primarily breast and lung tumors since the most common malignancies that metastasize to the pleural fluid are, in order of frequency, lung and breast. Our chromosome panel was successful in detecting breast and lung neoplasms with sensitivities of 100% and 75%, respectively. Moreover, 15 of the 24 tumors that FISH alone failed to detect were not covered by our panel, namely, 1 patient with acute myelocytic leukemia, 1 with renal cell carcinoma, 1 with cervical, 1 with chrondrosarcoma, 2 gastric,2 with ovarian, 2 with skin, 2 with colon and 3 with NSCLC. Indeed, it will be a challenge to be borne out of subsequent studies to determine how large of a panel of chromosomes will be required to identify the variety of neoplasms that metastasize to the pleural cavity.
The survival rate correlated predominant aneusomy for chromosomes 11 has been previously reported in breast cancer and NSCLC. For breast cancer, patients with no exhibiting aneuploidy for chromosome 11 in cancerous effusions had a significantly shorter overall survival rate than did have patients who had aneuploidy . Other studies also found that poor prognosis was associated with aneusomy of chromosome 17 in breast cancer [22, 23]. In the present work, our results were consistent with the above-mentioned studies showing that aneuploidy for chromosome 17 was associated with poor prognosis in patients with malignant pleural effusions. But survival rate was not found to correlate with the predominant aneusomy for chromosomes 7 and 11.
We also observed in the present study that pleural fluids positive by the combined FISH and cytology analysis correlated with pleural invasion. These data suggest that there is a higher yield of malignant cells in the pleural fluid after the pleura is invaded by tumor. This is in agreement with recent studies performed using pleural lavages during pulmonary resections which have shown increased detection of positive pleural lavage cytology when there is parietal pleura invasion by malignancy . Combined cytology and FISH analysis were also more accurate in discerning exudative samples from patients with a primary malignancy. In general, exudative malignant pleural effusions have higher cell counts, lower glucose and pH levels, and are cytologically positive. But these criteria are far from absolute , and in our dataset, cytologic analysis only detected 57% of exudative pleural fluids from patients with a known primary malignancy compared with 64% for FISH alone and 79% for the chromosome and cytology assays combined.
Limitations of the present study include the small cohort size that precludes detailed analysis of specific tumor subtypes. Only descriptive, observational conclusions about the FISH analyses in patients with NSCLC and breast cancer can be made. Second, only FISH technology with 3 probes were used in this study. The patients mainly came from Chinese north areas. Therefore, it is need more study in a large patient group and varieties areas in China in order to figure out specific chromosome anomalies to specific tumors in large population. Finally, the patients with benign disease in this study only had 6 months of longitudinal follow-up to determine if they remained cancer free. That is usually insufficient time to allow preneoplastic events to progress to clinically detectable cancer. Despite our ability to detect tumour-associated aneuploidy in pleural fluid, the sensitivity of this assay would most likely be enhanced by designing separate chromosome panels for tumors originating from specific organs. Chromosome profiles of tumors from different organs do seem to be distinct [26, 27], but more validity studies are necessary before these profiles can be optimized for routine clinical diagnostic use. Improving the diagnostic performance of any test has the potential to alter medical practice, even if, as in this case, it simply means eliminating the need for repeated thoracentesis or other invasive procedures to secure the diagnosis of metastatic disease in a patient with a know primary tumor. Although a more sensitive test to diagnose patients earlier may have limited clinical value in extending survival in patients with metastatic disease, this assay may have important diagnostic implications particularly in patients who have unilateral pleural effusions as their principal presenting clinical sign. It is conceivable that chromosome profiles of the pleural fluid could also be used to identify the site of origin of the metastatic malignant cells in the chest. But this technology requires much validation before it becomes clinically widespread.
In conclusion, this study shows the feasibility of detecting tumour-associated aneuploidy from a variety of different malignancies in the pleural fluid of patients even if conventional cytologic analyses are negative. In addition, the FISH test may increase the sensitivity of conventional cytology without a corresponding decrease in specificity. The present results were obtained retrospectively and suggest that simple numeric FISH anomalies may be prognostic. Gainning of chromosome 17 in malignant pleural effusions is a marker of superior prognosis. This observation may prompt us to perform prospective studies in which chromosomal changes in malignancies will be correlated with survival rate. Larger translational studies will be needed to validate that molecular markers may improve the diagnostic yield of the current standard examination of pleural fluid.
Fluorescence in situ hybridization.
Financial support for this study was provided by Science Foundation of Heilongjiang Health Department (No.2011-157) and China Postdoctoral Science Foundation (No.106941).
- Cagle PT, Churg A: Differential diagnosis of benign and malignant mesothelial proliferations on pleural biopsies. Arch Pathol Lab Med. 2005, 129: 1421-1427.PubMedGoogle Scholar
- Chhieng DC, Ko EC, Yee HT, Shultz JJ, Dorvault CC, Eltoum IA: Malignant pleural effusions due to small-cell lung carcinoma: a cytologic and immunocytochemical study. Diagn Cytopathol. 2001, 25: 356-360. 10.1002/dc.10011.View ArticlePubMedGoogle Scholar
- DeCamp MM, Jaklitsch MT, Mentzer SJ, Harpole DH, Sugarbaker DJ: The safety and versatility of video-thoracoscopy: a prospective analysis of 895 consecutive cases. J Am Coll Surg. 1995, 181: 113-120.PubMedGoogle Scholar
- Dawson AJ, Bal S, McTavish B, Tomiuk M, Schroedter I, Ahsanuddin AN, Seftel MD, Vallente R, Mai S, Cotter PD: Inversion and deletion of 16q22 defined by array CGH, FISH, and RT-PCR in a patient with AML. Cancer Genet. 2011, 204: 344-347. 10.1016/j.cancergen.2011.05.005.View ArticlePubMedGoogle Scholar
- Hartmann L, Biggerstaff JS, Chapman DB, Scott JM, Johnson KR, Ghirardelli KM, Fritschle WK, Martinez DL, Bennington RK, de Baca ME: Detection of genomic abnormalities in multiple myeloma: the application of FISH analysis in combination with various plasma cell enrichment techniques. Am J Clin Pathol. 2011, 136: 712-720. 10.1309/AJCPF7NFLW8UAJEP.View ArticlePubMedGoogle Scholar
- Panzeri E, Conconi D, Antolini L, Redaelli S, Valsecchi MG, Bovo G, Pallotti F, Vigano P, Strada G, Dalpra L, Bentivegna A: Chromosomal aberrations in bladder cancer: fresh versus formalin fixed paraffin embedded tissue and targeted FISH versus wide microarray-based CGH analysis. PLoS One. 2011, 6: e24237-10.1371/journal.pone.0024237.View ArticlePubMedPubMed CentralGoogle Scholar
- Drieschner N, Rippe V, Laabs A, Dittberner L, Nimzyk R, Junker K, Rommel B, Kiefer Y, Belge G, Bullerdiek J, Sendt W: Interphase fluorescence in situ hybridization analysis detects a much higher rate of thyroid tumors with clonal cytogenetic deviations of the main cytogenetic subgroups than conventional cytogenetics. Cancer Genet. 2011, 204: 366-374. 10.1016/j.cancergen.2011.03.008.View ArticlePubMedGoogle Scholar
- Herrera JC, Isaza LF, Ramirez JL, Vasquez G, Muneton CM: Detection of chromosome 17 aneuplody and TP53 gene deletion in a broad variety of solid tumors by dual-color fluorescence in situ hybridization (FISH). Biomedica. 2010, 30: 390-400.View ArticlePubMedGoogle Scholar
- Drach J, Angerler J, Schuster J, Rothermundt C, Thalhammer R, Haas OA, Jager U, Fiegl M, Geissler K, Ludwig H, Huber H: Interphase fluorescence in situ hybridization identifies chromosomal abnormalities in plasma cells from patients with monoclonal gammopathy of undetermined significance. Blood. 1995, 86: 3915-3921.PubMedGoogle Scholar
- Eastmond DA, Schuler M, Rupa DS: Advantages and limitations of using fluorescence in situ hybridization for the detection of aneuploidy in interphase human cells. Mutat Res. 1995, 348: 153-162. 10.1016/0165-7992(95)90003-9.View ArticlePubMedGoogle Scholar
- Fiegl M, Massoner A, Haun M, Sturm W, Kaufmann H, Hack R, Krugmann J, Fritzer-Szekeres M, Grunewald K, Gastl G: Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH). Br J Cancer. 2004, 91: 558-563. 10.1038/sj.bjc.6601942.View ArticlePubMedPubMed CentralGoogle Scholar
- Fiegl M, Zojer N, Kaufmann H, Mullauer L, Schuster R, Huber H, Drach J: Hyperdiploidy and apparent aneusomy in mesothelial cells from non-malignant effusions as detected by fluorescence in situ hybridization (FISH). Cytometry. 1999, 38: 15-23. 10.1002/(SICI)1097-0320(19990215)38:1<15::AID-CYTO3>3.0.CO;2-C.View ArticlePubMedGoogle Scholar
- Runyon BA, Montano AA, Akriviadis EA, Antillon MR, Irving MA, McHutchison JG: The serum-ascites albumin gradient is superior to the exudate-transudate concept in the differential diagnosis of ascites. Ann Intern Med. 1992, 117: 215-220.View ArticlePubMedGoogle Scholar
- Cora T, Acar H, Ceran S, Bodur S: Analysis of chromosomes 9 and 11 aneuploidy frequency in pleural effusion of patients with and without malignancy: interphase FISH technique. Cancer Biol Ther. 2005, 4: 248-251. 10.4161/cbt.4.2.1573.View ArticlePubMedGoogle Scholar
- Fiegl M, Haun M, Massoner A, Krugmann J, Muller-Holzner E, Hack R, Hilbe W, Marth C, Duba HC, Gastl G, Grunewald K: Combination of cytology, fluorescence in situ hybridization for aneuploidy, and reverse-transcriptase polymerase chain reaction for human mammaglobin/mammaglobin B expression improves diagnosis of malignant effusions. J Clin Oncol. 2004, 22: 474-483.View ArticlePubMedGoogle Scholar
- Fiegl M, Kaufmann H, Zojer N, Schuster R, Wiener H, Mullauer L, Roka S, Huber H, Drach J: Malignant cell detection by fluorescence in situ hybridization (FISH) in effusions from patients with carcinoma. Hum Pathol. 2000, 31: 448-455. 10.1053/hp.2000.6550.View ArticlePubMedGoogle Scholar
- Fehm T, Sagalowsky A, Clifford E, Beitsch P, Saboorian H, Euhus D, Meng S, Morrison L, Tucker T, Lane N: Cytogenetic evidence that circulating epithelial cells in patients with carcinoma are malignant. Clin Cancer Res. 2002, 8: 2073-2084.PubMedGoogle Scholar
- Massoner A, Augustin F, Duba HC, Zojer N, Fiegl M: FISH cytogenetics and prognosis in breast and non-small cell lung cancers. Cytometry B Clin Cytom. 2004, 62: 52-56.View ArticlePubMedGoogle Scholar
- Krishnamurti U, Hammers JL, Atem FD, Storto PD, Silverman JF: Poor prognostic significance of unamplified chromosome 17 polysomy in invasive breast carcinoma. Mod Pathol. 2009, 22: 1044-1048. 10.1038/modpathol.2009.61.View ArticlePubMedGoogle Scholar
- Watters AD, Going JJ, Cooke TG, Bartlett JM: Chromosome 17 aneusomy is associated with poor prognostic factors in invasive breast carcinoma. Breast Cancer Res Treat. 2003, 77: 109-114. 10.1023/A:1021399923825.View ArticlePubMedGoogle Scholar
- Tiainen M, Tammilehto L, Rautonen J, Tuomi T, Mattson K, Knuutila S: Chromosomal abnormalities and their correlations with asbestos exposure and survival in patients with mesothelioma. Br J Cancer. 1989, 60: 618-626. 10.1038/bjc.1989.326.View ArticlePubMedPubMed CentralGoogle Scholar
- McManus DT, Patterson AH, Maxwell P, Hamilton PW, Anderson NH, Caughley LM, Toner PG: Interphase cytogenetics of chromosomes 11 and 17 in fine needle aspirates of breast cancer. Hum Pathol. 1999, 30: 137-144. 10.1016/S0046-8177(99)90267-8.View ArticlePubMedGoogle Scholar
- Ichikawa D, Hashimoto N, Hoshima M, Yamaguchi T, Sawai K, Nakamura Y, Takahashi T, Abe T, Inazawa J: Analysis of numerical aberrations of specific chromosomes by fluorescent in situ hybridization as a diagnostic tool in breast cancer. Cancer. 1996, 77: 2064-2069. 10.1002/(SICI)1097-0142(19960515)77:10<2064::AID-CNCR15>3.0.CO;2-T.View ArticlePubMedGoogle Scholar
- Vicidomini G, Santini M, Fiorello A, Parascandolo V, Calabro B, Pastore V: Intraoperative pleural lavage: is it a valid prognostic factor in lung cancer?. Ann Thorac Surg. 2005, 79: 254-257. 10.1016/j.athoracsur.2004.06.115. discussion 254–257View ArticlePubMedGoogle Scholar
- Doelken P: Management of pleural effusion in the cancer patient. Semin Respir Crit Care Med. 2010, 31: 734-742. 10.1055/s-0030-1269833.View ArticlePubMedGoogle Scholar
- Chen M, Ye Y, Yang H, Tamboli P, Matin S, Tannir NM, Wood CG, Gu J, Wu X: Genome-wide profiling of chromosomal alterations in renal cell carcinoma using high-density single nucleotide polymorphism arrays. Int J Cancer. 2009, 125: 2342-2348. 10.1002/ijc.24642.View ArticlePubMedPubMed CentralGoogle Scholar
- Horlings HM, Lai C, Nuyten DS, Halfwerk H, Kristel P, van Beers E, Joosse SA, Klijn C, Nederlof PM, Reinders MJ: Integration of DNA copy number alterations and prognostic gene expression signatures in breast cancer patients. Clin Cancer Res. 2010, 16: 651-663. 10.1158/1078-0432.CCR-09-0709.View ArticlePubMedGoogle Scholar