Human cardiac tissue in a microperfusion chamber simulating extracorporeal circulation - ischemia and apoptosis studies

Table 1 Representing the effect of cardioplegia on calcium homoeostasis.

Group	Cp/rep in min	Ratio initial	Ratio final	Δ-ratio Ratio_final-ratio_initial	n =
Control, non-cp + rep	20/7	1.59 ± 0.04	1.65 ± 0.02	0.06 *	5
Control, non-cp + rep	40/13	1.68 ± 0.02	1.83 ± 0.02	0.12 *	5
Control, non-cp + rep	60/20	1.52 ± 0.01	1.64 ± 0.05	0.12 *	5
Cp + rep	20/7	1.57 ± 0.01	1.58 ± 0.01	0.01	5
Cp + rep	40/13	1.56 ± 0.02	1.56 ± 0.01	0	5
Cp + rep	60/20	1.60 ± 0.01	1.62 ± 0.01	0.02	5

Experimental protocol comparing calcium homoeostasis in the control group (non-cardioplegia and reperfusion) versus the cardioplegia and reperfusion group. Time sets were 20/7, 40/13 and 60/20 min. Δ-ratio was calculated as ratio_final-ratio_initial. Ratio values of n = 5 experiments are noted as mean ± SEM. Significant (p < 0.05) calcium homoeostasis disarrangements were detected in the control group (marked with stars).

ISSN: 1749-8090