Figure 3

VEGF overexpression exerts increased capillary density, reduced CMs apoptosis, promoted CMs proliferation two weeks after viral injection. A, Representative histological micrographs of the LV myocardium stained with hematoxylin-eosin B, vWF immunostaining to identify capillary density C, Histological identification of apoptotic CMs for TUNEL D, Ki-67 staining to detect CMs proliferation E, Quantitative analysis of the cross-sectional area of CMs, and ratios of capillary, TUNEL positive nuclei and Ki-67 positive nuclei to myocyte in LV myocardium (Bars = 100 μm). *P < 0.05, TAC vs. Sham; †P < 0.05, VEGF vs. TAC.