Figure 4

Morphology diversity under distint treated factors (Magnification 10 × 10). SV VSMC: a) DMEM/F12 b) 10%FBS c) 10 ng/ml PDGF-BB. ITA VSMC: d) DMEM/F12 e) 10%FBS f) 10 ng/ml PDGF-BB. a) and d): Cells grew with long and thin shape, refractive index of the cell edge increased. Cells line more compactly, and gradually formed a multil-overlapping, bundles, polarity, similar to the organic assembled VSMCs of the blood vessel wall. b) and e): Cells grew with typical "hill and valley" pattern, from multil-overlapping and non-cell regions. c) and f): Polarity of the cells disappeared, and proliferation were enhanced,form many ultra-overlapping regions.