- Poster presentation
- Open Access
Volume-activated chloride channel exists in CD133+ lung cancer stem cells
© Mao and Xu; licensee BioMed Central Ltd. 2013
- Published: 11 September 2013
- Public Health
- Stem Cell
- Lung Cancer
- Flow Cytometry
- Cardiac Surgery
The study is to research if volume-activated chloride channel (VACC) existed in tumor stem cells.
CD133+ cells were purified by magnetic cell separation (MACS) system from non-small cell lung cancer (NSCLC) cell line A549. The purity was detected by flow cytometry. VACC was recorded with whole-cell patch clamp.
The purity of CD133+ cells isolated from A549 by MACS was 92.14%. And VACC was recorded in the 22/40(55%) CD133+ cells of A549 by whole-cell patch clamp.
VACC exist in CD133+ lung cancer stem cells.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.