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Volume-activated chloride channel exists in CD133+ lung cancer stem cells

Background

The study is to research if volume-activated chloride channel (VACC) existed in tumor stem cells.

Methods

CD133+ cells were purified by magnetic cell separation (MACS) system from non-small cell lung cancer (NSCLC) cell line A549. The purity was detected by flow cytometry. VACC was recorded with whole-cell patch clamp.

Results

The purity of CD133+ cells isolated from A549 by MACS was 92.14%. And VACC was recorded in the 22/40(55%) CD133+ cells of A549 by whole-cell patch clamp.

Conclusion

VACC exist in CD133+ lung cancer stem cells.

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Correspondence to Z Mao.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mao, Z., Xu, X. Volume-activated chloride channel exists in CD133+ lung cancer stem cells. J Cardiothorac Surg 8, P135 (2013). https://doi.org/10.1186/1749-8090-8-S1-P135

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Keywords

  • Public Health
  • Stem Cell
  • Lung Cancer
  • Flow Cytometry
  • Cardiac Surgery