Skip to content

Advertisement

  • Poster presentation
  • Open Access

Plasma cholinesterase activity in children with infective endocarditis

Journal of Cardiothoracic Surgery20138 (Suppl 1) :P177

https://doi.org/10.1186/1749-8090-8-S1-P177

  • Published:

Keywords

  • Endocarditis
  • Cholinesterase
  • Infective Endocarditis
  • Positive Culture
  • Positive Blood Culture

Background

Although pre-existing heart disease in children is the most frequent predisposing factor for infective endocarditis (IE), we reporte 6 children of IE with no apparent pre-existing cardiac disease.

Methods

Our study included six patients with acquired infection from central catheter; the microbial pathogens were Streptococcus viridans (n=3) and Staphyloccocus aureus (n=3). The diagnosis of IE was based on positive blood culture (2 positive cultures of blood samples drawn > 12 hours), evidence of endocardial involvement (positive ECHO), measuring raised erythrocyte sedimentation rate and plasma cholinesterase (PChE) activity. We measured PChE activity (µmol min-1ml-1) in children with IE (group A, n=6) and in IE-free children (group B, n=10). For determination of PChE activity vein blood samples were collected and stored at -20°C until analyzed. PChE activity was determined by the spectrophotometric method of Ellman using butyryltiocholine as the substract (Sigma Chemical Co., St.Luis, Mo, USA). Statistical analysis was made by Student’s t-test.

Results

In group A of examined children with IE we measured decreased PChE activity (1.89 ± 0,24) relation to patients of control group B (3,97 ± 0,20).

Conclusion

Our study indicates that PChE activity can be also an important parameter for the diagnosis of IE.

Authors’ Affiliations

(1)
Department of Anesthesiology, Reanimatology and Intensive Care, Children's Hospital Zagreb, Zagreb, Croatia

Copyright

Advertisement