Fig. 4
Myocardial apoptosis was detected by TUNEL staining. A The apoptotic cells were indicated by TUNEL-positive nuclei (red staining). B Quantification of the number of TUNEL positive nuclei per mm2 area. Data are reported as mean ± S.D. for three independent experiments (***, P < 0.001; unpaired Student’s t test). Abbreviations: NHSP, normothermic hyperkalemic simultaneous blood perfusion; NNSP, normothermic normokalemic simultaneous blood perfusion