Fig. 4

Confirmation of the attachment of transplanted cell sheet at forty-eight hours (a–c) and twenty-eight days (d–f) after transplantation. Left panels are SMC-Tx (a, d), middle panels are DFB-Tx (b, e), and right panels are Untreated (c, f). Red signal, PKH stained cell sheet; blue signal, DAPI for nuclear staining. Microphotographs of transplanted cell sheet forty-eight hours after transplantation that has been stained with TUNEL assay in SMC-Tx (g) and DFB-Tx (h) groups. Numbers of TUNEL-positive grafted cell sheets (i). *p < 0.01 vs. SMC-Tx. Each groups n = 5. Scale bar = 500 μm (a–f), 50 μm (g, h). SMC-Tx, smooth muscle cell sheet transplantation group; DFB-Tx, dermal fibroblast sheet transplantation group; Untreated, untreated group