Table 5 Decellularization protocol for replacement heart valves in eligible full-text articles

Bechtel et al. 2005^a [39]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Bechtel et al. 2008^a [40]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Bibevski et al. 2017^a [41]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Boethig et al., 2019^b [42]

0.5% sodium deoxycholate (Sigma Chemical Company, St Louis, Missouri, USA) + 0.5% sodium dodecylsulfate (Carl Roth GmbH, Karlsruhe, Germany); 0.9% NaCl (washing)

Penicillin-streptomycin (100 IU/mL)^e

20 °C (treatment); 4 °C (storage)

Brown et al. 2010^a [43]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Brown et al. 2011^a [44]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Burch et al. 2010^a [45]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Cebotari et al. 2011 [46]

0.5% sodium deoxycholate + 0.5% sodium dodecylsulfate (treatment); 0.9% NaCl (washing)

Penicillin-streptomycin (100 IU/mL)^e

20 °C (treatment); 4 °C (storage)

da Costa et al. 2005^c [47]

1 to 2% sodium deoxycholic acid (treatment); PBS in decreasing concentrations (rinsing); 70% ethanol; pulsating PBS (repeating rinsing)

37 °C (storage for 24 h); 20 °C (treatment)

da Costa et al. 2007 [33]

10% dimethyl sulphoxide + 10% fetal bovine serum; 0.9% NaCl (thawing) + 10% fetal bovine serum (gradual dilution); continuous shaking over 24 h with either 1% sodium deoxycholic acid + 80% ethanol OR 0.1% sodium dodecylsulfate; nutrient solution (harvesting)

Cefoxitin (240 μg/mL) + lincomycin (120 μg/mL) + vancomycin (50 μg/mL) + polymyxin B (100 μg/mL)

4 °C (treatment); Storage within −150 °C liquid nitrogen vapour; 42 to 50 °C (thawing)

da Costa et al. 2018^d [48]

0.1% sodium dodecylsulfate (treatment); PBS (storage)

37 °C (shaking); 4 °C (storage up to 90 days)

Etnel et al. 2018^d [49]

0.1% sodium dodecylsulfate (treatment); PBS (storage)

37 °C (shaking); 4 °C (storage up to 90 days)

Konuma et al. 2009^a [50]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Ruzmetov et al. 2012^a [51]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Sarikouch et al. 2016 [34]

0.5% sodium deoxycholate (Sigma Chemical Company, St Louis, Missouri, USA) + 0.5% sodium dodecylsulfate (Carl Roth GmbH, Karlsruhe, Germany) (treatment); PBS (6 cycles of washing, 12 h each)

Penicillin-streptomycin (100 IU/mL)^e

4 °C (storage)

Sievers et al. 2003^a [52]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)

Tavakkol et al. 2005^a [53]

Hypotonic sterile water solution (treatment); multi-day washout period with isotonic neutral buffer solution (unspecified)

Ribonuclease + deoxyribonuclease

Antibiotic + antimycotic (unspecified)