Fig. 3

The effect of Gli signaling on EMT and p-AKT expression in NSCLC cell lines. a Western blots of A549 and H1975 cells, subjected to siRNA treatment. Cells were given control siRNA (100 nM) or Gli1 + Gli2 siRNA (100 nM each) for 48 h prior to total protein extraction. Expression of Gli1, Gli2, N-cadherin, Vimentin, p-AKT and AKT expression were examined, with GAPDH serving as an internal control. b Western blots of H1975 cells, treated with DMSO (control), N-Shh (0.5 mg/mL), GANT61(15umol/L), or GANT61(15umol/L) and N-Shh (0.5 mg/mL) stimulation, for 24 h prior to total protein extraction. Expression of Gli1, Gli2, N-cadherin, p-AKT and AKT expression were examined, with GAPDH serving as an internal control