Fig. 5

AKT inhibition reduces cell invasion and EMT in NSCLC cell lines. a Transwell invasion assay of H1975 lung cancer cells, treated with DMSO (control), N-Shh (0.5 mg/mL), AKT-i(1 μmol/L), or AKT-i(1 μmol/L) with N-Shh (0.5 mg/mL) stimulation. Representative images captured by a light microscope are shown (100x), average of 5 picture fields at 100x total magnification. b Quantification of transwell invasion assays, expressed as percentage of wound in comparison to 0 h (p-values of < 0.001 was indicated as ***). c Western blots of EMT marker was examined in H1975 (N-cadherin), with GAPDH as a loading control. Cells were pretreated with DMSO, N-Shh (0.5 mg/mL), AKT-i(1 μmol/L), or AKT-i(1 μmol/L) with N-Shh (0.5 mg/mL) stimulation prior to protein extraction