Fig. 4

The effect of DMF on the expression of inflammatory cytokines in the serum and hippocampal tissues of LPS-induced ALI model. The mRNA expression of TNF-α (A), IL-1β (B) and IL-6 (C) were measured using qRT-PCR. The proteins expression levels of TNF-α and IL-1β were analyzed using the Western blot analysis (D). The proteins expression of TNF-α and IL-1β were quantified by compared with GAPDH (E, F). The proteins expression levels of p-NF-κB and p-IKBα were analyzed using the Western blot analysis (G). The ratios of p-NF-κB and p-IKBα were quantified by compared with GAPDH (H).The values are presented as means ± SD. **P < 0.01 versus control group; ^#P < 0.05, ^##P < 0.01 versus ALI group