Fig. 2

FBXL19 overexpression attenuates Spn-induced lung injury in pneumonia immature mice. Immature mice were intravenously injected with FBXL19 lentivirus overexpression vectors (FBXL19), with lentivirus empty vector (NC) as the negative control. Then, a pneumonia immature model (SPN) was established by Spn induction. A–B: FBXL19 expression levels in lung tissues were determined by RT-qPCR (A) and Western blot assay (B); C The survival rates of immature mice within 14 days were analyzed by Kaplan–Meier plots; D Bacterial loads in BALF in each group; E Lung injury scoring and representative images of H&E staining; F The lung wet/dry weight ratios; G MPO activity in lung tissues; H Contents of IL-1β, IL-6, and IL-10 in lung tissues examined by ELISA. C N = 18, A, B and D–H N = 6. Experiments were repeated 3 times. Data in panel C were analyzed using the log-rank test, data in panel D were analyzed by the t test, and data in panels A, B, E, F, G, and H were analyzed using one-way ANOVA, followed by Tukey’s multiple comparison test. BALF broncho-alveolar lavage fluid, MPO myeloperoxidase