Fig. 4

FBXL19 ubiquitinates and degrades FOXM1. A Ubiquitination levels of FOXM1 in lung tissues; B: The binding of FBXL19 to FOXM1 was tested by the Co-IP assay; MLE-12 cells were infected with FBXL19 lentivirus overexpression vectors (FBXL19), with lentivirus empty vector (NC) as the negative control; C, D FBXL19 expression levels in cells were determined by RT-qPCR (C) and Western blot assay (D); MLE-12 cells were treated with MG132, with dimethylsulfoxide (DMSO) as the negative control; E Ubiquitination levels of FOXM1 in cells; F–G FOXM1 expression levels in cells were determined by Western blot assay (F) and RT-qPCR (G). Experiments were performed in triplicate. Data in panel C were analyzed using the t test, and data in panels D, F, and G were analyzed using one-way ANOVA, followed by Tukey’s multiple comparison test