Fig. 5

FOXM1 overexpression counteracts the protective role of FBXL19 overexpression in lung injury in pneumonia immature mice. Immature mice were intravenously injected with FOXM1 lentivirus overexpression vectors (FOXM1), with lentivirus empty vector as the negative control (NC). Then, a pneumonia immature model (SPN) was established by Spn induction. A, B FOXM1 expression levels in lung tissues were determined by RT-qPCR (A) and Western blot assay (B); C: The survival rates of immature mice within 14 days were analyzed by Kaplan–Meier plots; D Bacterial loads in BALF in each group; E: Lung injury scoring and representative images of H&E staining; F The lung wet/dry weight ratios; G: MPO activity in lung tissues; H Contents of IL-1β, IL-6, and IL-10 in lung tissues examined by ELISA. C N = 18, A, B and D–H N = 6. Experiments were repeated 3 times. Data in panel C were analyzed by the log-rank test, and data in panels A, B, D, E, F, G, and H were analyzed using one-way ANOVA, followed by Tukey’s multiple comparison test. BALF: broncho-alveolar lavage fluid, MPO: myeloperoxidase