Table 1 Properties of primers used in RT-PCR

EpCAM

60 °C

1 (R² = 0.98)

Forward

5′- TGATCCTGACTGCGATGAGAG-3′

Reverse

5′- CTTGTCTGTTCTTCTGACCCC-3′

CEACAM5

60 °C

0.99 (R² = 0.97)

Forward

5′-TCTTGGCTGATTGATGGGAAC-3′

Reverse

5′-CACTGGCTGAGTTATTGGCCT-3′

CK19

55 °C

0.99 (R² = 0.93)

Forward

5′-AACGCCGAGCTAGAGGTGA-3′

Reverse

5′-GGATGGTCGTGTAGTAGTGCC-3′

GAPDH

55–60 °C

1 (R² = 0.99)

Forward

5′-AGGGCTGCTTTTAACTCTGGT-3′

Reverse

5′-CCCCACTTGATTTTGGAGGGA-3′