Fig. 4

Nrf2 inhibition partially invalidates protective function of DEX on HG-induced cardiomyocyte injury. (A) The protein levels of nuclear Nrf2, GPX4, Bcl2 and Bax were determined by Western blot; (B) Cell viability was detected using the MTT method; (C) Cell apoptotic rate was estimated by flow cytometry; (D) Total SOD activity and MDA levels; (E) Fe²⁺ concentration; (F) DCFH-DA was used to assess ROS levels. Three replicates were guaranteed in cell experiments. Mean ± standard deviation was introduced to represent data. The comparison between 2 groups was made using the independent sample t test. * p < 0.05, ** p < 0.01